Journal: Journal of Cell Science

Article Title: Interplay between nuclear survivin and the PRC2 complex and its impact on H3K27me3-directed transcriptional repression

doi: 10.1242/jcs.264572

Figure Lengend Snippet: Hypoxia increases expression of EZH2, H3K27me3 and survivin. (A) Immunoblots of WCEs from U2OS, HeLa and MRC5 lines cultured under normoxic or hypoxic environments (24 h). Blots were immunoprobed with anti-EZH2, anti-H3K27me3 and anti-survivin antibodies. Anti-Hif1a used to prove the hypoxic state had been induced, and anti-tubulin was used as a loading control. (B–D) Quantification of immunoblots represented in A from three independent experiments demonstrating that EZH2, H3K27me3 and survivin are all more abundant under hypoxia. Data presented are means±s.d. * P <0.05, ** P <0.01, *** P <0.001 (two-way ANOVA with Tukey's multiple comparisons post test).

Article Snippet: Human cervical cancer cells (HeLa), human bone osteosarcoma cells (U2OS), retinal pigment epithelial cells (RPE) and human breast cancer cells (MCF7) were originally from ATCC.

Techniques: Expressing, Western Blot, Cell Culture, Control

Journal: Scientific Reports

Article Title: The C16orf87 protein is a subunit of the MIER corepressor complex controlling embryonic development and cell migration

doi: 10.1038/s41598-026-50740-7

Figure Lengend Snippet: C16orf87 partially mediates HDAC1 and MIER1 protein interactions. ( A ) C16orf87 interacts with the HDAC and MIER proteins. Volcano plot of the IP-MS experiment showing identified proteins interacting with the Flag-C16orf87 protein in HeLa cells. An adjusted P -value cut-off of 0.05 and a log2 fold change cut-off of 2 were used. Data are shown from a biological triplicate experiment. ( B ) Lack of C16orf87 does not change HDAC and MIER protein accumulation. Soluble Panc-01 WT (WT) and Panc-01 KO (KO) whole-cell lysates were analyzed by WB with the indicated antibodies. ( C ) C16orf87 partially mediates HDAC1 and MIER1 interaction. Co-immunoprecipitation of Flag-HDAC1 from siRNA (siC16 and siScr) and pcDNA3-Flag-HDAC1-transfected HeLa cells. Isolated proteins were analyzed by WB with the indicated antibodies. An arrowhead indicates the migration of the MIER1 protein isoforms, whereas an asterisk indicates the migration of the C16orf87 isoforms. ( D ) HDAC1 interacts weakly with C16orf87 in vitro. GST (as a control) and GST-HDAC1 pull-down with bacterially purified 8 × His-tagged C16orf87(Wt, 5 × C > A, 1–130, and 5 × C > A/1–130) proteins. An asterisk indicates a degradation product/partially translated GST-HDAC1. Proteins were detected with the anti-His and anti-GST antibodies.

Article Snippet: Human pancreatic cancer cell lines Panc-01 (ATCC, CRL-1469) and MiaPaCa-2 (ATCC, CRL-1420), mouse skeletal muscle cell line C2C12 (ATCC, CRL-1772), and human cervical cancer cell line HeLa S3 (ATCC, CCL-2.2) were used in this study.

Techniques: Protein-Protein interactions, Immunoprecipitation, Transfection, Isolation, Migration, In Vitro, Control, Purification

Journal: Frontiers in Pharmacology

Article Title: Genistein inhibits the replication of enterovirus A71

doi: 10.3389/fphar.2026.1787050

Figure Lengend Snippet: Effect of the genistein on EV71 replication in HeLa cell. (A) Cytotoxicity of genistein on HeLa cells. HeLa cells were treated with serially diluted genistein for 24 h, after which the cell viabilities were measured by cell counting kit-8 assay (CCK8). N = 3 replicates in each concentration. (B) HeLa cells were infected with EV71 at a MOI of 5 for 2 h. At 2 h post-infection, cells were treated with 10% DMEM or genistein (GET, 75 μM) for 22 h. Morphology was recorded using an inverted microscope. N = 3 independent experiments. (C) At 2 h post-infection, cells were treated with genistein (0, 50, 75, 100 μM) for 22 h. Western blot analysis of VP1 was performed. Tubulin was used a loading control. (D) Corresponding to (C) the gray value ratio of the VP1 protein band to the Tubulin protein band was shown. Data were presented as the mean ± SD (N = 3 independent experiments); statistical analysis was performed by one-way ANOVA test with Tukey’s multiple comparisons test. (*** P < 0.001). (E) At 2 h post-infection, cells were treated with genistein (75 μM) for 22 h. Relative mRNA levels of viral genome were measured using VP1 primers. GAPDH was used as a housekeeping gene. Data were presented as the mean ± SD (N = 3 independent experiments); statistical analysis was performed using T-test. (*** P < 0.001). (F) At 2 h post-infection, cells were treated with genistein (0, 50, 75, 100 μM) for 22 h. Virus from the supernatant was collected for plaque assay analysis. Plaques in RD cells were recorded after diluting the supernatant 5000-fold. (G) Corresponding to (F) data were presented as the mean ± SD (N = 3 independent experiments); statistical analysis was performed using one-way ANOVA test with Tukey’s multiple comparisons test. (** P < 0.01, *** P < 0.001). (H) At 2 h post-infection, cells were treated with genistein (75 μM) for 22 h. Intracellular and supernatant progeny virions were titrated using RD cells to determine TCID50/ml. Data were presented as the mean ± SD (N = 3 independent experiments); statistical analysis was performed using a T-test (*** P < 0.001).

Article Snippet: Human rhabdomyosarcoma RD cells (CCL-136) and human cervical cancer HeLa cells (CCL-2TM) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Cell Counting, Concentration Assay, Infection, Inverted Microscopy, Western Blot, Control, Virus, Plaque Assay

Journal: Frontiers in Pharmacology

Article Title: Genistein inhibits the replication of enterovirus A71

doi: 10.3389/fphar.2026.1787050

Figure Lengend Snippet: Genistein inhibited autophagy. (A) Cherry-GFP-LC3 plasmid was transfected into HeLa cells, at 24 h post transfection, HeLa cells treated with rapamycin (Rapa; 8 nM), genistein (GET; 75 μM), or both rapamycin (8 nM) and genistein (75 μM) for 24 h. Immunofluorescence analysis was recorded. Scale bars = 10 μm. (B) Corresponding to (A) , the GFP-LC3 positive dots was counted by ImageJ (N = 3, *** P < 0.001, ns: no significant difference). (C) RD cells were treated with rapamycin (8 nM) or together with doses of genistein (GET, 0, 50, 75, 100 μM) for 24 h. Then cells were collected for Western blotting analysis. (D,E) is calculated according to (C) . Data were presented as the mean ± SD (N = 3 independent experiments); Statistical analysis of was performed using one-way ANOVA with Tukey’s multiple comparisons test. (ns: No significant difference, * P < 0.05, ** P < 0.01,*** P < 0.001). (F) RD cells were infected with EV71 at a MOI of 1 for 2 h, at 2 h post infection, cells were treated with genistein (GET, 0, 50, 75, 100 μM) for 22 h. At 24 h post infection, cells were collected for Western blotting analysis. (G,H) is calculated according to (F) . The quantification of relative VP1 and LC3II/Tubulin were shown. Data were presented as the mean ± SD (N = 3 independent experiments); Statistical analysis of was performed using one-way ANOVA with Tukey’s multiple comparisons test. (* P < 0.05, ** P < 0.01,*** P < 0.001). (I) RD cells were infected with EV71 at a MOI of 1 for 2 h, at 2 h post infection, cells were treated with 3-MA (2, 4, 6 mM) for 22 h. At 24 h post infection, cells were collected for Western blotting analysis. (J,K) is calculated according to (I) . The quantification of relative VP1 and LC3II/Tubulin were shown. Data were presented as the mean ± SD (n = 3 independent experiments); Statistical analysis of was performed using one-way ANOVA with Tukey’s multiple comparisons test (* P < 0.05, ** P < 0.01,*** P < 0.001).

Article Snippet: Human rhabdomyosarcoma RD cells (CCL-136) and human cervical cancer HeLa cells (CCL-2TM) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Plasmid Preparation, Transfection, Immunofluorescence, Western Blot, Infection

Journal: Frontiers in Pharmacology

Article Title: Genistein inhibits the replication of enterovirus A71

doi: 10.3389/fphar.2026.1787050

Figure Lengend Snippet: Genistein induces G2/M arrest, which inhibits viral replication. (A,B) HeLa cells were treated with genistein (GET, 75 μM) or 10% DMEM for 24 h. Flow cytometry was used to analyze the proportion of cells in G0/G1, G2/M, and S phases of the cell cycle. (G2/M of Con vs. GET, ### P < 0.001). (C,D) HeLa cells were infected with EV71 at a MOI of 5 for 2 h, then treated with genistein (75 μM) for 22 h. Flow cytometry analysis was performed to determine the distribution of cells in G0/G1, G2/M, and S phases. (S of Mock + Con vs. EV + Con, ** P < 0.01; G2/M of Mock + Con vs. Mock + GET, ### P < 0.001; G2/M of EV + Con vs. EV + GET, ### P < 0.001). (E,F) RD cells were infected with EV71 at a MOI of 1 for 2 h, then treated with genistein (75 μM) for 22 h. Flow cytometry analysis was performed to determine the distribution of cells in G0/G1, G2/M, and S phases. (S of Mock + Con vs. EV + Con, *** P < 0.001; G2/M of Mock + Con vs. Mock + GET, ### P < 0.001; G2/M of EV + Con vs. EV + GET, ### P < 0.001) (G–M) HeLa cells were treated with genistein (0, 50, 75, 100 μM) for 24 h. Western blotting was used to analyze the relative protein levels of CDK4, CyclinB1, CDK1, CyclinE1, CDK2, and CyclinD1. Data were presented as mean ± SD (N = 3 independent experiments); statistical analysis was conducted using one-way ANOVA with Tukey’s multiple comparisons test. (ns: no significant difference; * P < 0.05; ** P < 0.01; *** P < 0.001). (N,O) HeLa cells were treated with nocodazole (Noco; 20 nM) or 10% DMEM for 24 h. Flow cytometry was used to analyze the proportion of cells in G0/G1, G2/M, and S phases. (S of Mock + Con vs. EV + Con, ** P < 0.01; G2/M of Mock + Con vs. Mock + Noco, ### P < 0.001; G2/M of EV + Con vs. EV + Noco, ### P < 0.001). (P,Q) HeLa cells were infected with EV71 at a MOI of 5 for 2 h; at 2 h post-infection, nocodazole (20 nM) was administered, and at 24 h post-infection, cells were collected for Western blotting. Tubulin served as the loading control. Data were presented as mean ± SEM (N = 3 independent experiments); statistical analysis was performed using a T-test. (*** P < 0.001).

Article Snippet: Human rhabdomyosarcoma RD cells (CCL-136) and human cervical cancer HeLa cells (CCL-2TM) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Flow Cytometry, Infection, Western Blot, Control

Journal: Nature Genetics

Article Title: High-content CRISPR activation screens identify synthetically lethal RNA-based mechanisms to sensitize cancer cells to targeted T cell cytotoxicity

doi: 10.1038/s41588-026-02561-7

Figure Lengend Snippet: a , MYC ORF-based overexpression sensitizes cervical cancer cells to T cell cytotoxicity. The fraction of surviving HPV + cervical cancer cells ( y axis) in coculture with E7 TCR T cells (1:1, 48 h), is shown for CaSki cells transduced to express a control, BID or MYC ORF (mean ± s.d., n = 3 technical replicates per ORF). **** P < 0.001, ordinary one-way ANOVA, Dunnett’s multiple-comparison test. b , Fold-change in A375 cell viability after 24-h treatment with FasL (200 ng ml −1 ), shown for A375 cells with ORF-based overexpression of different sensitizing hits compared to A375 cells with a control ORF (mean ± s.d., n = 3–6 technical replicates per ORF). **** P < 0.0001, ** P < 0.01, ordinary one-way ANOVA, Dunnett’s multiple-comparison test, compared to control cells. c , UMAP of Perturb-seq data of control (NTC) and MYC CRISPRa cells, colored based on: (1) culture conditions, (2) sgRNA and MYC expression level, (3) the expression of the MYC GA signature and (4) the expression of the CRISPRa-resistance hit GAS7 (log 2 1p-transformed tp100k). d , Expression of MYC GA signature in control (NTC) cells and cells with MYC CRISPRa sgRNAs, further stratified based on MYC expression in monoculture (left) and coculture (right). The number of cells in each group is shown in parentheses ( n ). Boxplots: the middle line shows the median, the box edges show the 25th and 75th percentiles and the whiskers show the most extreme points that do not exceed ±1.5× the interquartile range (IQR). Further outliers are marked individually with circles (minima or maxima). **** P < 0.0001, one-tailed Student’s t -test.

Article Snippet: The cervical cancer cell line CaSki (ATCC, cat. no. CRL-1550) was cultured in Roswell Park Memorial Institute (RPMI) medium with GlutaMAX (Gibco, cat. no. 72400-047) supplemented with 10% FBS and 1× Pen–Strep.

Techniques: Over Expression, Control, Comparison, Expressing, Transformation Assay, One-tailed Test